Chlorophyll a/b-Binding Protein Gene Expression in Cotton

Chlorophyll a/b-binding protein gene expression in cotton.

To investigate the regulation of genes encoding CAB proteins in cotton (Gossypium hirsutum) and identify potentially useful promoters, we determined the structure and expression of two genes (Lhcbl*Z and Lhcb3*l) encoding type I and type 111 proteins of the LHCII subfamily (Table I). Northern analyses of the gene expression pattem in cotton demonstrated substantial LHCII transcript accumulation...

IL-23 Gene and Protein Expression in Childhood Asthma

Background: Asthma is the chronic inflammation of airways characterized by eosinophilic infiltration, mucus overproduction, airway hyper-responsiveness and airway remodeling. These changes are induced mostly by cytokines which are produced by T helper (Th) 2 cells. Recently, the role of interleukin-23 (IL-23) in the pathogenesis of adultallergic asthma has been studied. Objective: To explore IL...

Mycobacterium tuberculosis HspX/EsxS Fusion Protein: Gene Cloning, Protein Expression, and Purification in Escherichia coli

Background: The purpose of this study was to clone, express, and purify a novel multidomain fusion protein of Micobacterium tuberculosis (Mtb) in a prokaryotic system. Methods: An hspX/esxS gene construct was synthesized and ligated into a pGH plasmid, E. coli TOP10 cells were transformed, and the vector was purified. The vector containing the construct and pET-21b (+) plasmid were digested ...

Expression and Distribution of Calcium-Binding Protein S100P in Human Placenta during Pregnancy

Structure and expression of three light-harvesting chlorophyll a/b-binding protein genes in Arabidopsis thaliana.

The genome of Arabidopsis thaliana is exceedingly small, in part because it lacks the large middle repetitive DNA component characteristic of other plants. In this paper we have characterized a member of the low copy DNA component: the gene family for the light-harvesting chlorophyll a/b-protein. This gene family is unusual in that it contains far fewer members than the 7-16 coding sequences fo...